Journal: Gels

Article Title: Tannic Acid-Enhanced Gelatin-Based Composite Hydrogel as a Candidate for Canine Periodontal Regeneration

doi: 10.3390/gels11080650

Figure Lengend Snippet: Metabolic activity of L929 cell cultures in basal medium (control) and exposed to 5% ( a ), 10% ( b ) and 25% ( c ) extract of tannic–gelatin-based hydrogel (Gel), Gel with hydroxyapatite 2% (Gel-HA), Gel with graphene oxide 7.5% (Gel-GO), and Gel with graphene oxide 7.5% and hydroxyapatite 2% (Gel-GO-HA) for 24 and 48 h. * Significantly different from the cultures performed in basal conditions ( p ≤ 0.05).

Article Snippet: Europe, Zwijndrecht, Belgium), Graphene Oxide (GO, >95 wt% pure, 90% GO particles < 5–7 μm, 50% < 2–4 μm, and 10% < 1–2 μm, reflecting controlled distribution of lateral particle sizes; Graphenea, San Sebastian, Spain), Tannic acid (TA, Mw 1701.20 g/mol; C 76 H 52 O 46 ) and ammonia solution (NH 4 OH, 25%) (Sigma-Aldrich, St. Louis, MO, USA), Citric acid monohydrate (C 6 H 8 O 7 ·H 2 O, 99.5%) and calcium nitrate tetrahydrate ((Ca(NO 3 ) 2 ·4H 2 O), 99%) (Riedel-deHaën, Seelze, Germany), and Ammonium hydrogen phosphate ((NH 4 ) 2 ·HPO 4 , 99%; Merck, Darmstadt, Germany).

Techniques: Activity Assay, Control

Journal: Gels

Article Title: Tannic Acid-Enhanced Gelatin-Based Composite Hydrogel as a Candidate for Canine Periodontal Regeneration

doi: 10.3390/gels11080650

Figure Lengend Snippet: ( a , b ) Metabolic activity on days 3, 7, and 14 and ( c ) immunocytochemical staining of F-actin cytoskeleton (green) and nucleus (blue) on day 3 of canine periodontal ligament-derived cell cultures in basal medium (control) and exposed to 5% and 10% extracts of tannic–gelatin-based hydrogel (Gel), Gel with hydroxyapatite 2% (Gel-HA), Gel with graphene oxide 7.5% (Gel-GO), and Gel with graphene oxide 7.5% and hydroxyapatite 2% (Gel-GO-HA). Significant differences were not observed compared to cultures performed in basal conditions. Bar = 100 µm.

Article Snippet: Europe, Zwijndrecht, Belgium), Graphene Oxide (GO, >95 wt% pure, 90% GO particles < 5–7 μm, 50% < 2–4 μm, and 10% < 1–2 μm, reflecting controlled distribution of lateral particle sizes; Graphenea, San Sebastian, Spain), Tannic acid (TA, Mw 1701.20 g/mol; C 76 H 52 O 46 ) and ammonia solution (NH 4 OH, 25%) (Sigma-Aldrich, St. Louis, MO, USA), Citric acid monohydrate (C 6 H 8 O 7 ·H 2 O, 99.5%) and calcium nitrate tetrahydrate ((Ca(NO 3 ) 2 ·4H 2 O), 99%) (Riedel-deHaën, Seelze, Germany), and Ammonium hydrogen phosphate ((NH 4 ) 2 ·HPO 4 , 99%; Merck, Darmstadt, Germany).

Techniques: Activity Assay, Staining, Derivative Assay, Control

Journal: Gels

Article Title: Tannic Acid-Enhanced Gelatin-Based Composite Hydrogel as a Candidate for Canine Periodontal Regeneration

doi: 10.3390/gels11080650

Figure Lengend Snippet: ( a , b ) ALP activity and ( c , d ) ALP histochemical staining of canine periodontal ligament-derived cell cultures exposed to 5% ( a ) and 10% ( b ) extracts of tannic–gelatin (Gel; control), gel with hydroxyapatite 2% (Gel-HA), gel with graphene oxide 7.5% (Gel-GO), and gel with graphene oxide 7.5% and hydroxyapatite 2% (Gel-GO-HA) for 7 and 14 days. * Significantly different from the cultures exposed to the Gel extract ( p ≤ 0.05). Bar = 500 µm.

Article Snippet: Europe, Zwijndrecht, Belgium), Graphene Oxide (GO, >95 wt% pure, 90% GO particles < 5–7 μm, 50% < 2–4 μm, and 10% < 1–2 μm, reflecting controlled distribution of lateral particle sizes; Graphenea, San Sebastian, Spain), Tannic acid (TA, Mw 1701.20 g/mol; C 76 H 52 O 46 ) and ammonia solution (NH 4 OH, 25%) (Sigma-Aldrich, St. Louis, MO, USA), Citric acid monohydrate (C 6 H 8 O 7 ·H 2 O, 99.5%) and calcium nitrate tetrahydrate ((Ca(NO 3 ) 2 ·4H 2 O), 99%) (Riedel-deHaën, Seelze, Germany), and Ammonium hydrogen phosphate ((NH 4 ) 2 ·HPO 4 , 99%; Merck, Darmstadt, Germany).

Techniques: Activity Assay, Staining, Derivative Assay, Control